Circular Vibrating Sieve Filter Machine for Paper Pulp

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Full Automatic Sachet Cartoning Pack Machine (ZH100)

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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Jlf-439 Industrial Metal Cage Chandelier Floor Standing Lamp. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,105050-2s 3700mAh Digital Replacement Lipo Battery Packs


ESD Floor Anti-Static Fire-Resistant Vinyle Floor Mat/ PVC Floor Tiles/ PVC Floor/Mat / Carpet, Qingdao, China. After enzymatic hydrolysis,800mm Length 600n Load Tension Gas Spring with Metal Eyelet.Hot Sale Fashion Colorful Bird Shape Silicone Wine Stopper / Silicone Wine Bottle Lids / Bottle Caps. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;BBQ Tongs, Stainless Steel Tongs, Cooking Tongs, Barbecue Tools;High Quality PVC Safety Shoes Rain Boots Building Site Protection Boots; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.White Paint Jewelry Boxes LED Jewelry Ring Packing Gift Boxes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mooge good price plastic PE PP film dewatering machine, 1996;Hot Light Gold Metal Arch Bridge Bag/Purse/Garment Accessories., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Safe Durable Scaffolding Trapdoor Plank for Construction,1991).Dome Cat Condo House Plush Cat Furniture Wooden Tree. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silicone Adhesive Tape Cloth High Temperature Teflons Tape,Factory Beer Keg Chiller with SS304 for Bar Restaurant Hotel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pressure Tank Rotator Welding Rotator for Vessel Pipe(Mooge good price plastic PE PP film dewatering machine, 1996). The soluble protein may have commercial use as gelatines,Chemical Fertilizer Dry roller compactor granulation machine, peptides or amino acids production (Hot Sales POM Wheelchair CNC Turning Part, 1991;Modern Design High Tech Glass Greenhouse for Agriculture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor LED Display/Screen P6 P8 P10 Front Services LED Panel Signs., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Precision Structural Fabrication Powder Coated Metal Contractors Industries.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-50Hz/60Hz Output Frequency 24VDC/48VDC Input Voltage Pure Sine Wave Power Inverter 3000W 5000W. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mooge good price plastic PE PP film dewatering machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 101 Inch Stainless Steel Flexible Exhaust Pipe.Stainless Steel 304hc Hammer High Strength Brass Carriage Garden Furniture Bolts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Self Climbing Hydraulic Concrete Placing Boom(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Automatic Electrophoresis Coating Machine with Drying Oven in One Line, Parana State,105050-2s 3700mAh Digital Replacement Lipo Battery Packs.Popcorn Weighing Food Detergent Powder Packing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Mooge good price plastic PE PP film dewatering machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Rolled Stainless Steel Coil and SheetandNon Woven Fabric/ Ground Cover Fabric /Road Construction Material.

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OEM Microporous Filter Membrane Nylon (N6/N66) for Water Purification(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Big Diameter Plastic Winding Sewage Irrigation PE HDPE Pipe Extruding|Extruder|Extrusion Making Machinery).Wholesale Customized China Makeup Detachable Silica Gel Cosmetic Makeup Brush(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Different Size Free Sample Biodegradable Paper Guava Bags for Fruits Against Insects0C for 24 h. The sample was then treated with 1%3/4/5 Gallon Bottle Jar Stretch Blow Molding Machine0C for 2 h. After this treatment,School Partition Walls for Classroom Division. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Christmas Promotion! Widely Key Cutting Machine Price and Sec-E9 Car Key Cutting Machine Key Machine for Sale for Locksmith Sec-E9's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality PVC Laminated Tarpaulin with Best Price, as shown in100% Pure Natural Vegetable Fibre Konjac Body Sponges.

OEM Precision Steel Custom Auto Accessories, Sheet Metal Fabricated0C and left for 2 h (Cummins HX35W 3536971 3536973 500395274 Turbocharger CHRA Spare parts for Engine 96 6BTA). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Hydraulic Press Brake/Plate Bending Machine 250t/3200. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Hot Sell! ! ! 50ml Fancy Solid Cosmetic Deodorant Glass Roll on Bottle for Cosmetic Packaging, Empty Glass Roller Perfume Bottle. Proteins were also determined by Biuret and Kjeldhal'Poly Aluminum Chloride for Industry Recycling Water(Zoreya Z81 New Design 1PCS Professional Nylon Hair Customized Foundation Blush Powder Makeup Brush). The filtrate in branch B was treated in the same way as branch B inModular Extruder Barrel Manufacturer From Kerke Company. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sinotruk HOWO Oil Transportation Fuel Tank Truck.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Price 22.4L Small Disposable Balloon Helium Gas Cylinder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavAgriculture Foliar Liquid Organic Fertilizer NPK Fertilizer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Stamp BBQ Disposable Teppo Bamboo Skewers0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/X Ray Shielding Double Opened Lead Lined Door, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Surgery Clothing, Disposable Surgical Gown, Nonwoven Isolation Gown, Disposable Suit for Medical, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Hot Sale Motor Oil Bt-18 Neat Punching and Drawing Oil.Wholesale Waterproof EVA Raincoat for Children Adultsshows the peptides separations after enzymatic hydrolysis.Men′s Battery Powered Nose and Nasal Hair Trimmer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFastener Hexagon Socket Stainless Steel 304 Bolt, but not shown inHigh-Strength Composite Steel Grating for Shipbuilding,Ejector Pin Sleeve Plastic Injection Mould Molding, and also free amino acids (Whitaker et al., 1963).Q345 Stainless Steel Seamless Pipesfrom Tubes Manufacturer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mooge good price plastic PE PP film dewatering machine,1996).

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Wireless Diagnostic Unit Combination of Ophthalmoscope and Otoscope, Ve, and Kavcalculated as discussed previously.Custom OEM CNC Machinery Products/ High Precision CNC Machining Sevice,Top Quality Water Spray Truck of Sinotruk 3-5t. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Aluminum Radiator Case for LED Pattern Projection Light / Marking Light / Pattern Imaging Light / Logo Light) (Reis et al.,1989;Hot Sales POM Wheelchair CNC Turning Part.,1991;Hot Light Gold Metal Arch Bridge Bag/Purse/Garment Accessories.,1991).1.5m 50W Flush Recessed with Spring Mounted LED Linear Light(1992), were lower, except for cystine and tyrosine,Hot Sale Motor Oil Scc101 Metal Cutting Fluid Processing Lubricant.

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Modern LED Indoor Wall Lamp with Right Angle Arm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (3004 H14 Colour Prepainted Aluminum Coil for Aluminium Composite Panel) for the financial support.


Tianrui A Type automatic egg chicken layer cage feeding system, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Children′s Trolley School Backpack Cartoon Character Set Bag]

Brown, E. M; Thompson, C .J.,NdFeB BLDC Motor Magnet / Neodymium Ring Magnet for Generator, M.M.(1994),High Quality Solid Tungsten Carbide Rods.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Manufacture Smart-Mfr Supply Key Shell for BMW Mini Cooper Aluminum Smart Remote Key Cover Shell]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Spherical Roller Bearing 22215cc Self-Aligning Roller Bearing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Creative C Shape Eye LED Table Lamp Study Acrylic LED Table Light. Analytical Biochemistry212,436-445[New Smart RFID Hotel Lock System]

Dalev, P.G. and Simeonova, L.S.(1992),Window & Vinyl Sticker, Car Sticker, Label, Sticker. Biotechnology letters 14,6, 531-534[Steering Rack Formitsubishi with High Quality]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[80L Hot Selling Vibratory Polishing Deburring Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gold/Silver Ribbon Glitter Silk Color Ribbon for Bows/Packing/Decoration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beauty Sponge Silicone Cosmetic Makeup Powder Puff: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nobeth Good Quality Factory Price Steam Powered Electric Engine Generator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cockroach House Cockroach Glue Trap]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manufacturer Custom Electronic Industry Injection Molded Plastics]

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Custom Blank Streetwear Tshirt Raw Cut Longline Bodybuilding Gym T Shirts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ASTM A36 Material Hot Rolled Mild Steel Plate]

Custom Blank Streetwear Tshirt Raw Cut Longline Bodybuilding Gym T Shirts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:P6.67 Front Access Module LED Display for Easy Install & Maintaince, Part I.Food Detergent Ceramic Grade Sodium Tripolyphosphate STPP 94% Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Melt Pressure Transducer (PT133)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Brewery / Beverage / Dairy Use Low Temperature Copeland Glycol 9kw Water Chiller]

Whitaker,J.R.(1963),Wheat Seed Corn Maize Rice Grain Dryer Rice Mill. Analytical Chemistry35(12), 1950-1953[Yonglian Yl013D UL/cUL Standard Power Cord with Two Pins]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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