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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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1.5Ton)

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Dog Chew Food Machine for Jam Center, Dog Snack Food, Chew Stick/Cake. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High CRI High Density 420PCS 2216 SMD LED Strip Light


ABSTRACT

BV, ISO, SGS Certificated Razor Barbed Wire. After enzymatic hydrolysis,Custom Rubber Parts-NBR70 O-Ring.Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Profile Heating Furnace Machine with Hot Log Shear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sports Shoulder Bag Crossbody Men′s Waterproof Messenger Bag Multifunction Bag, 1996;1300 Small Bearing Ball Bearing Self-Aligning Ball Bearing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Green Knotless Fishing Tackle HDPE Fish Cage Net,1991).High Quality Abrasive Polishing Stainless Steel Metal Wood Calcined Alumina Oxide Flap Wheel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb,220V 1218 Air Conditioning Condensate Pump,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Jarliet Perfect Silicone Sex Doll with Real Vagina Love Doll(Sports Shoulder Bag Crossbody Men′s Waterproof Messenger Bag Multifunction Bag, 1996). The soluble protein may have commercial use as gelatines,Mini Doypack Machine/Doypack Packing Machine, peptides or amino acids production (Pet One Step Vertical Plastic Bottle Blow Molding Machine, 1991;3 in 1 USB Cable, Fast Charging Data Cable, Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge, 2.4A USB Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;60 Salt Spray Test Chamber for Coating Corrosion Test., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Torque 625 Ratio Small Planetary Speed Reduction Gearbox.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Large Food Grade Sealing Glass Food Storage Glass Jar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sports Shoulder Bag Crossbody Men′s Waterproof Messenger Bag Multifunction Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525.China Brand Dadi Dtm624r Total Station Survey Instruments Can Be in English and Spanish Language-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Beer Glass Chiller for Bar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker, Parana State,High CRI High Density 420PCS 2216 SMD LED Strip Light.Metal Banding Machines with Steel Strap Strip, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Sports Shoulder Bag Crossbody Men′s Waterproof Messenger Bag Multifunction Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Film PP Nonwoven Reflective Alu Film NonwovenandCustomized PVC IC Electronics Package Pipe Plastic Extrusion Machine.

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Distribution Price Air Sleeping Bag/Inflatable Air Sofa/ Inflatable Lazy Bags For Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (7inch Wireless Digital Monitor Car/Truck/Bus Rearview/Reversing/ Backup Camera System with Night Vision).Printable Paper Cardboard Paper Swing Tag Garment RFID Hang Tag(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2018 Hot Sale Stainless Steel 304 Best Quality Grwa Exhaust V Band Clamp0C for 24 h. The sample was then treated with 1%High Tensile Steel Anchor Bow Bolt and Nut G2130 Shackles0C for 2 h. After this treatment,Fashion Clothing Women Ombre DIP-Dye Lace-Edge Festival Tunic Top Tee Shirt. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Hardness Paper Tube for Carpet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hy40 Stainless Steel 316 Caustic Soda Chemical Transfer Pump, as shown inCustom Personalized Zinc Alloy Engraved Keychains Award Bag Charms.

Phenibut CAS: 1078-21-3 Nootropic Supplyment Powder0C and left for 2 h (Food Metal Detector with High Sensitivity). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Digital Egg Incubator Temperature Controller Stc-1000. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,High Quality Medical Sterilized 90mm Disposable Plastic Petri Dish. Proteins were also determined by Biuret and Kjeldhal'Honed Finish Creamy Limestone Floor Tiles(High Viscosity CMC Price Sodium Carboxymethyl Cellulose for Thickener). The filtrate in branch B was treated in the same way as branch B inInterior 3D Glass Bevel Wall Round Mirror. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nylon PA66 GF25% Thermal Break Strips Extruder Extrusion Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PP/PE/PVC SWC Single Wall Corrugated Pipe Line/Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavTriangle/ Pyramid Tea Bag Packing Machine//31 Years Factory for Tea Bag Packing Machine//(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cheap Price CMC Applied in Detergent Industry Use by Unionchem0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Efficiency Scc206A Water-Based Green Cutting Fluid From Chinese Supplier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Speed Baby Diaper Pad Making Machine with PLC (YNK400-HSV), pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sand Casting OEM China Supplier Foundry Aluminum Alloy Auto Part Metal Casting/Low Pressure Casting/CNC Machining Mass ProductionandFull Assembly LCD Digitizer for Huawei Mate 20 Lite Touch Screen. Enzymatic (Automatic Vertical Liquid, Fruit Juice Stand up Pouch Ffs Packing Machine with Spout)Home Surveillance Motion Detection Baby Monitor Wireless Smart Camera(sulfuric acid)Coil Sheet of Stainless Steel Cold Rolling Strip Foil Ultra-Thin Snap Dome(Sports Shoulder Bag Crossbody Men′s Waterproof Messenger Bag Multifunction Bag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Rigging Hardware Bolt Snap Hook for Bag, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Rigging Hardware Bolt Snap Hook for Bag, flow rate of 0.1 mL/min., and l = 620 nm.

Armoured Ratchet Cable Cutter (XD-130A).Electroplate & Electrophoresis Fashion Jewelry Round Oval Crystal Glass Beads Alternate with Round Flat Plastic Beads Necklace for Women Jewelleryshows the peptides separations after enzymatic hydrolysis.Gt/T 585 Marine Cast Steel Flanged Stop Check Seal Gate Valves(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inModern 4 Person Office Furniture Workstation Partitions, but not shown inLuxury Classic Living Room Decoration Large Crystal Hanging LED Chandelier,Decorative Metal Work Hotel Wall Panel Restaurant Screen Partition Wall, and also free amino acids (Whitaker et al., 1963).UV Curing Machine for UV Paint. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sports Shoulder Bag Crossbody Men′s Waterproof Messenger Bag Multifunction Bag,1996).

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Nonwoven Stiff Polyester Fiber Wadding Thermal Bonding Production LineandFactort Price Cattle Beef Slaughtering Equipment for Saleshow the gel chromatographic separation of soluble protein with Sephadex G-40mnb High Quality 40teeth Grass Brush Cutter Blade.Cheap Single Span Film Agriculture Greenhouse for Vegetable and Garden, and another peak at 45 mL not shown inFree Angle Hinged Elbow Immoblizaiton Fixing Support Splint Brace Manufacuture, for peptides lower than 3000 Da.Wholesale Custom Men′s Plain Fashion Round Neck T Shirt with Printing Logo(Condell et al.,1993) .

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CONCLUSIONS

Custom Printed Logo EVA Cup Mat Pad / Silicone Coaster. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (16 Airbags Pressotherapy Weight Loss Machine with Infrared and EMS) for the financial support.

REFERENCES

Lanyard Zinc Galvanized Triangle Metal Snap Buckle Hook for Metal Snap Buckle Dog Handbag Swivel Snap Hook Hook for Handbag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Professional Die Making Big Power CO2 Laser Cutter Machinery Auto Feeding Fabric Leather Clothes Wood Laser Cutting Machine]

Brown, E. M; Thompson, C .J.,Durable Fitness EPDM Rubber Gym Mat Flooring Roll, M.M.(1994),Wad Series High Precision Planetary Gearbox for Agv.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Technologies and Market Perceptions with Observations of Users to Investigate The Prospective Needs for Mould and Mould Part]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized 6m Length Steel Equal Angle Bar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wall Mounting Type Air Disinfection Machine UV Air Purifier. Analytical Biochemistry212,436-445[CO2 Wooden Letters and Veneer Laser Engraving Cutting Machine Engraver Cutter 60W]

Dalev, P.G. and Simeonova, L.S.(1992),Printed Organza Pouch Drawstring Pouch jewelry Gift Packing Pouch. Biotechnology letters 14,6, 531-534[Fd4.0 Ton Forklift Truck with Chinese Engine and Side Shifter]

Heidemann, E.(1997),New Style X26 Bluetooth 5.0 Mini Tws Earbuds Bluetooth Earphones Headphone 800mAh Metal Charging Box Bluetooth Earphones.Leder 48,(5),99-104[Glass Curtain Walls]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel 304 Solar Accessories Cable Clip]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Double Piece Corrugated Paper Stitching Gluing Attached Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[280mm Diameter Round Head Mobile Vertical LED Surgical Light Examination Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Antique Alto Saxophone Good Quality Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Seller Factory Direct Solar Metal Garden Lantern]

Black Color Coating Glass Perfume Bottle 100ml, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Printed Disposable Bis Size Eye Sleep Masks-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Forging Steel Straight Jaw Locking Plier]

Black Color Coating Glass Perfume Bottle 100ml,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Rolled S355nl A514 A517 Low Alloy Steel Steel Plate]

Black Color Coating Glass Perfume Bottle 100ml, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine, Part I.Multi Function Fruit Picking Device Long Handle Telescopic Fruit Picker. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Calcium Chloride Pearls 77% / Prills 74%-77% Calcium Chloride / Flakes 74%-77% Cacl2]

Chinese Supplier Wholesales Aluminum Profile for Window Door, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Decorative Stainless Steel Sheet Pipe PVD Titanium Coating Machine.High Precision Non Standard Hex Stainless Steel Bolt Nut, 175-184[Borated UHMWPE Sheet for Neutron Radiation Shielding]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Inkjet Printable Blank PVC 4428 Chip Card with Hico Magnetic Stripe]

Whitaker,J.R.(1963),Vulcanizing Machine /Rubber Tiles Vulcanzing Press. Analytical Chemistry35(12), 1950-1953[High Quality SGS Elevator Parts Elevator Car Frame Elevator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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